DEVELOPMENT OF VALIDATED RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF TAMSULOSIN HYDROCHLORIDE AND FINASTERIDE FROM oral contraceptive DOSAGE FORM Susheel John Varghese, Leela Madhuri Pola, K.Murugavel, and T.K.Ravi. College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore 641044, Tamilnadu. E-Mail: velmpharm24@gmail.com A precise, accurate and rapid RP-HPLC method acting has been developed and validated for the coinciding estimation of Tamsulosin Hydrochloride and Finasteride in tablet dosage form. Chromatography was carried on RP 18e Hiber RT (250 × 4.6) chromatography column using Water:Methanol (30:70%) v/v as mobile phase at a full stop rate 0.7 ml/min and the effluent was monitored at 225 nm. The belongings times of Tamsulosin Hydrochloride and Finasteride were 5.4 and 15.4 minutes respectively. The regulate of detection for Tamsulosin Hydrochloride and Finasteride were fix to be 15 and 50 ng/ml respectively and their limit of quantification were launch to be 100 and 1250 ng/ml respectively.
Tamulosin Hydrochloride and Finasteride were found to be linear in the range of 0.3 to 10 µg/ml and 3.75 to 125 µg/ml respectively. The developed method was validated in terms of linearity, accuracy, precision, specificity, limit of detection, and limit of quantification as per ICH guidelines. System suitability parameters like number of theoretical plates (N), florescence asymmetry factor (As), and resolution (Rs), etc. were studied and it was found that all results were within limits. The proposed method was successfully applied for simultaneous determination of drugs in tablets. Keywords: Tamsulosin Hydrochloride, Finasteride, High Performance Liquid Chromatography, cooccurring determination. If you want to get a full essay, order it on our website:
OrderessayOrder your essay at
Orderessay and get a 100% original and high-quality custom paper within the required time frame.
No comments:
Post a Comment
Note: Only a member of this blog may post a comment.